(2006) Eastern blotting has been used to refer to the detection of fusion proteins through complementation.The method is similar to southwestern blot. This could be seen as similar to a Southern blot, however the interaction is between a DNA molecule (the aptamer) and a protein, rather than two DNA molecules. (2005) Eastern blot has been used to describe a blot of proteins on polyvinylidene fluoride membrane where the probe is an aptamer rather than an antibody.Since this is essentially a western blot, the charge reversal was used to dub this method an eastern blot. (2002) Eastern blot has also been used to describe an immunoblot performed on proteins blotted to a polyvinylidene fluoride membrane from a PAGE gel run with opposite polarity.The method is essentially far-eastern blot. This method has also been discussed in later work by the same group. The membrane is then probed with antibodies for epitopes of interest. The oxidized protein is then treated with a complex mixture, generating a new conjugate on the membrane.
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